# Fluorescence Spectroscopy Analyzer

> Fluorescence spectroscopy skill for quantum dot characterization, FRET measurements, and photoluminescence analysis

- Skill: `a5c-ai/fluorescence-spectroscopy-analyzer` (Agent Skill)
- Install (CLI): `npx skillmds@latest add a5c-ai/fluorescence-spectroscopy-analyzer`
- Raw SKILL.md: https://api.skillmd.com/api/skills/a5c-ai/fluorescence-spectroscopy-analyzer/raw
- Safety review: pending (external: skill-scanner PASS, skillspector PASS)
- Works with: Claude Code, Claude.ai, OpenAI Codex
- Category: Coding & Dev Tools
- Author: a5c-ai (https://skillmd.com/u/a5c-ai)
- Updated: 2026-09-09
- Page: https://skillmd.com/skills/a5c-ai/fluorescence-spectroscopy-analyzer

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# Fluorescence Spectroscopy Analyzer

## Purpose

The Fluorescence Spectroscopy Analyzer skill provides comprehensive photoluminescence characterization of nanomaterials, enabling analysis of emission properties, quantum yields, and energy transfer processes.

## Capabilities

- Excitation/emission spectra
- Quantum yield calculation
- Fluorescence lifetime measurements
- FRET efficiency calculation
- Photostability assessment
- Single-particle fluorescence

## Usage Guidelines

### Fluorescence Analysis

1. **Spectral Measurements**
   - Acquire excitation and emission spectra
   - Correct for instrument response
   - Identify emission maxima

2. **Quantum Yield**
   - Use integrating sphere or reference
   - Account for reabsorption
   - Report with standard

3. **Lifetime Measurements**
   - Use TCSPC for time-resolved
   - Fit decay curves
   - Identify multiple components

## Process Integration

- Multi-Modal Nanomaterial Characterization Pipeline
- Nanosensor Development and Validation Pipeline
- Nanoparticle Drug Delivery System Development

## Input Schema

```json
{
  "sample_id": "string",
  "excitation_wavelength": "number (nm)",
  "measurement_type": "steady_state|time_resolved|quantum_yield",
  "reference_standard": "string (optional)"
}
```

## Output Schema

```json
{
  "emission": {
    "peak_wavelength": "number (nm)",
    "fwhm": "number (nm)",
    "stokes_shift": "number (nm)"
  },
  "quantum_yield": {
    "value": "number",
    "method": "string",
    "reference": "string"
  },
  "lifetime": {
    "tau1": "number (ns)",
    "amplitude1": "number",
    "tau2": "number (ns, optional)",
    "chi_squared": "number"
  }
}
```