# Bio Clip Seq Binding Site Annotation

> Annotate CLIP-seq binding sites to genomic features including 3'UTR, 5'UTR, CDS, introns, and ncRNAs. Use when characterizing where an RBP binds in transcripts.

- Skill: `majiayu000/bio-clip-seq-binding-site-annotation` (Agent Skill, multi-file: 2 files)
- Install (CLI): `npx skillmds add majiayu000/bio-clip-seq-binding-site-annotation`
- Raw SKILL.md: https://api.skillmd.com/api/skills/majiayu000/bio-clip-seq-binding-site-annotation/raw
- Safety review: pending
- Works with: Claude Code, Claude.ai, OpenAI Codex
- Category: Coding & Dev Tools
- Author: majiayu000 (https://skillmd.com/u/majiayu000)
- Updated: 2026-09-09
- Page: https://skillmd.com/skills/majiayu000/bio-clip-seq-binding-site-annotation

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# Binding Site Annotation

## Using ChIPseeker (R)

```r
library(ChIPseeker)
library(TxDb.Hsapiens.UCSC.hg38.knownGene)

txdb <- TxDb.Hsapiens.UCSC.hg38.knownGene

peaks <- readPeakFile('peaks.bed')
anno <- annotatePeak(peaks, TxDb = txdb)

plotAnnoPie(anno)
```

## Using BEDTools

```bash
# Annotate to UTRs
bedtools intersect -a peaks.bed -b 3utr.bed -wa -wb > peaks_3utr.bed
```

## Python Annotation

```python
import pandas as pd

def annotate_peaks(peaks_bed, annotation_gtf):
    '''Annotate peaks to genomic features'''
    # Load peaks and annotations
    # Intersect and categorize
    pass
```

## Related Skills

- clip-peak-calling - Get peaks
- genome-intervals - Interval operations