# Scientific Crispr Design

> CRISPR design skill. Guide RNA design, off-target analysis, CRISPR screen analysis, gene knockout/knock-in design, and editing efficiency prediction.

- Skill: `nahisaho/scientific-crispr-design` (Agent Skill, multi-file: 2 files)
- Install (CLI): `npx skillmds@latest add nahisaho/scientific-crispr-design`
- Raw SKILL.md: https://api.skillmd.com/api/skills/nahisaho/scientific-crispr-design/raw
- Safety review: pending
- Works with: Claude Code, Claude.ai, OpenAI Codex
- Category: Coding & Dev Tools
- Author: nahisaho (https://skillmd.com/u/nahisaho)
- Updated: 2026-09-17
- Page: https://skillmd.com/skills/nahisaho/scientific-crispr-design

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# CRISPR design

CRISPR design skill. Guide RNA design, off-target analysis, CRISPR screen analysis, gene knockout/knock-in design, and editing efficiency prediction.

## Use This Skill When

- Guide RNA design.
- Off-target analysis.
- CRISPR screen analysis.
- Gene knockout/knock-in design.
- Editing efficiency prediction.

## Required Inputs

- Research objective, decision target, or hypothesis.
- Available data, source constraints, and domain assumptions.
- Required outputs, success metrics, and deadline or reproducibility constraints.

## Workflow

1. Confirm scope, assumptions, and the exact artifact set to save.
2. Apply the narrowest domain method that answers the request with defensible evidence.
3. Save code, tables, figures, and intermediate outputs to files instead of chat-only output.
4. State limitations, uncertainty, and any validation or sensitivity checks performed.
5. Append skill selection, handoff I/O, and file writes to `logs/process-log.jsonl`.

## Deliverables

- `report.md`: concise method, results, interpretation, and file inventory in the user's language.
- `results/`: structured outputs, metrics, model artifacts, or extracted findings.
- `figures/`: English-only charts, diagrams, or panels when visual output is needed.
- `data/`: processed or derived datasets when transformation occurs.

## Quality Gates

- The selected method matches the scientific question and stated assumptions.
- Outputs are reproducible, saved to files, and traceable from inputs to conclusions.
- Missing data, uncertainty, bias, and hard limits are made explicit.
- `report.md` and `logs/process-log.jsonl` reference the generated artifacts.