# Biochemistry

> Analyzes biochemical processes, including enzyme kinetics, metabolic pathways, and biomolecule characterization, with practical techniques and examples.

- Skill: `neuralblitz/biochemistry` (Agent Skill)
- Install (CLI): `npx skillmds@latest add neuralblitz/biochemistry`
- Raw SKILL.md: https://api.skillmd.com/api/skills/neuralblitz/biochemistry/raw
- Safety review: PASS (external: skill-scanner PASS, skillspector PASS)
- Works with: Claude Code, Claude.ai, OpenAI Codex
- Category: AI & ML, Coding & Dev Tools
- Tags: Biochemistry, Biomolecules, Chromatography, Enzyme Kinetics, Mass Spectrometry, Metabolic Pathways, Spectroscopy
- License: MIT
- Author: NeuralBlitz (https://skillmd.com/u/neuralblitz)
- Updated: 2026-08-22
- Page: https://skillmd.com/skills/neuralblitz/biochemistry

---


## What I do

- Analyze chemical reactions and processes in living systems
- Study enzyme kinetics and catalytic mechanisms
- Investigate metabolic pathways and regulation
- Characterize biomolecules (proteins, nucleic acids, lipids, carbohydrates)
- Research protein structure-function relationships
- Apply biochemical techniques to solve biological problems

## When to use me

- When studying enzyme mechanisms and inhibition
- When analyzing metabolic pathways and disorders
- When characterizing biomolecules and their interactions
- When investigating cellular biochemistry and signaling
- When developing biochemical assays and diagnostics

## Key Concepts

### Major Metabolic Pathways

**Glycolysis**: Glucose → Pyruvate + ATP
**Citric Acid Cycle**: Acetyl-CoA oxidation + electron carriers
**Oxidative Phosphorylation**: ATP synthesis via electron transport
**Gluconeogenesis**: Glucose synthesis from non-carbohydrates
**Beta-Oxidation**: Fatty acid catabolism
**Photosynthesis**: Light reactions + Calvin cycle

### Enzyme Kinetics

```python
# Example: Michaelis-Menten kinetics
import numpy as np

def michaelis_menten(S, Vmax, Km):
    """
    Calculate reaction velocity.
    S: Substrate concentration
    Vmax: Maximum velocity
    Km: Michaelis constant
    """
    return (Vmax * S) / (Km + S)

def lineweaver_burk(S, v, Vmax, Km):
    """Linearize Michaelis-Menten for parameter estimation."""
    return 1/v, 1/S, 1/Vmax, -1/Km
```

### Key Techniques

- Spectroscopy: UV-Vis, fluorescence, circular dichroism
- Chromatography: HPLC, FPLC, affinity chromatography
- Electrophoresis: SDS-PAGE, native PAGE, 2D gel
- Mass spectrometry: MALDI-TOF, LC-MS
- Calorimetry: ITC, DSC
- Microscopy: Confocal, cryo-EM