# Opentrons Workflow

> Generate and understand Opentrons OT-2 workflows, including example well-plate patterns that use explicit aspirate and dispense operations. Use when selecting, setting up, or generating Opentrons workflow protocols.

- Skill: `pudap/opentrons-workflow` (Agent Skill, multi-file: 5 files)
- Install (CLI): `npx skillmds@latest add pudap/opentrons-workflow`
- Raw SKILL.md: https://api.skillmd.com/api/skills/pudap/opentrons-workflow/raw
- Safety review: pending
- Works with: Claude Code, Claude.ai, OpenAI Codex
- Category: Productivity
- Author: pudap (https://skillmd.com/u/pudap)
- Updated: 2026-09-17
- Page: https://skillmd.com/skills/pudap/opentrons-workflow

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# Opentrons Workflow

Use this skill for Opentrons OT-2 workflow guidance and protocol generation.

## Workflows

### Example P Shape (`example`)

Use for **creating a P-shaped liquid pattern on an Opentrons OT-2 destination plate**.

Capabilities:
- Generates an OT-2 Python protocol that dispenses into a fixed set of wells shaped like the letter `P`
- Uses explicit `pipette.aspirate(...)` and `pipette.dispense(...)` calls for each destination well
- Supports configurable source labware, destination labware, tip rack, pipette, deck slots, source well, and dispense volume
- Ends with `pipette.drop_tip()` so no tip remains attached

Use this workflow when:
- The user wants an example Opentrons workflow
- The task mentions making a `P` shape, letter pattern, or well-plate pattern using aspirate and dispense
- The workflow should demonstrate direct Opentrons liquid handling rather than optimization

Before running:
- Refer to: [example P shape](references/p-shape.md)
- Protocol generator: [scripts/p_shape.py](scripts/p_shape.py)
- Machine reference: [opentrons machine](../machine/references/opentrons-machine.md)
- Ask the user to confirm all deck slots before generating or running the protocol

## Critical Rules

1. Ask the user for source labware, destination labware, tip rack, pipette, mount, deck slots, source well, and dispense volume before generating a runnable protocol.
2. Do not assume deck slots.
3. Use explicit `pipette.aspirate(...)` and `pipette.dispense(...)` calls for the P-shape workflow; do not replace them with `transfer()` or `distribute()`.
4. Opentrons protocols must always end with no tip attached.
5. Do not run robot movement unless the user explicitly approves execution.