Ginkgo Cloud Lab
Overview
Ginkgo Cloud Lab (https://cloud.ginkgo.bio) provides remote access to Ginkgo Bioworks' autonomous lab infrastructure. Protocols are executed on Reconfigurable Automation Carts (RACs) -- modular units with robotic arms, maglev sample transport, and industrial-grade software spanning 70+ instruments.
The platform also includes EstiMate, an AI agent that accepts human-language protocol descriptions and returns feasibility assessments and pricing for custom workflows beyond the listed protocols.
The catalog is organized into Expression & Purification (in vitro / cell-free / E. coli / Pichia), Characterization & Assay, Method & Target Onboarding, and Specialty. Pick a protocol below, then read its reference file for inputs, outputs, the automated workflow, and ordering details.
Available Protocols
Expression & Purification - In vitro
| Protocol |
Readout |
Price |
Turnaround |
Status |
| IVT mRNA/circRNA Synthesis |
qPCR (mRNA or circRNA, 384-well) |
$99/sample |
up to 12 business days |
Certified |
Expression & Purification - Cell-free (E. coli CFPS)
| Protocol |
Readout |
Price |
Turnaround |
Status |
| Validate sequence expression |
Go/no-go titer + purity (up to 1800 bp) |
$39/sample |
up to 10 days |
Certified |
| Optimize expression conditions |
DoE across 24 conditions |
$199/sample |
up to 11 days |
Certified |
| Express + quantify (HiBiT) |
Luminescence, no purification |
$39/sample |
up to 11 days |
Certified |
| Express + purify (A280) |
Strep-tag, A280 yield |
$149/sample |
up to 11 days |
Certified |
| Express + purify minibinder |
Strep-tag, A280, LabChip |
$149/sample |
up to 11 days |
Certified |
| Express + purify (A280 + LabChip) |
Strep-tag, A280 + purity/size |
$159/sample |
up to 12 days |
Certified |
Expression & Purification - E. coli
| Protocol |
Readout |
Price |
Turnaround |
Status |
| Express + quantify (HiBiT) |
Luminescence (up to 384 constructs) |
$79/sample |
up to 3 weeks |
Certified |
| Express + purify (A280) |
His-tag, A280 yield |
$199/sample |
up to 3 weeks |
Certified |
| Express + purify minibinder |
His-tag, A280 yield |
$199/sample |
up to 3 weeks |
Certified |
| Express + purify (A280 + LabChip) |
His-tag, A280 + purity/size |
$209/sample |
up to 3 weeks |
Certified |
Expression & Purification - Pichia
| Protocol |
Readout |
Price |
Turnaround |
Status |
| Express + quantify (LabChip) |
Secreted protein, size/purity (up to 96) |
$89/sample |
up to 4 weeks |
Certified (New) |
Characterization & Assay
| Protocol |
Readout |
Price |
Turnaround |
Status |
| Express + thermal shift |
SYPRO Orange Tm (Tonset, TM1-3) |
$159/sample |
up to 12 days |
Certified |
| Detect enzymatic products (Echo-MS) |
Substrate/product by Echo-MS |
$44/sample |
up to 13 days |
Beta |
Method & Target Onboarding
| Protocol |
Readout |
Price |
Turnaround |
Status |
| Onboard Echo-MS method |
Calibration curve, LOD/LOQ |
$799/molecule |
up to 3 weeks |
Certified |
| Onboard SPR target |
Validated SPR capture method |
$1,399/target |
up to 4 weeks |
Beta |
Specialty
| Protocol |
Readout |
Price |
Turnaround |
Status |
| Generate fluorescent pixel art |
UV photo, 7-color E. coli palette |
$25/plate |
up to 7 days |
Beta |
Coming soon: Protein Expression and Binding Affinity Characterization (express + purify, then screen binding affinity against a target).
Choosing a Protocol
- Quick expressibility screen? Cell-free HiBiT ($39) or Validate sequence expression ($39).
- Need purified protein + yield? A280 tiers (cell-free or E. coli); add LabChip for purity/size.
- Difficult / membrane / disulfide / cofactor targets? Cell-free Optimize (24-condition DoE).
- Secreted or eukaryotic targets? Pichia expression.
- Screening de novo binders/minibinders? Cell-free or E. coli minibinder tiers, then SPR onboarding for kinetics.
- Enzyme activity / biocatalysis? Echo-MS enzymatic detection (onboard the analyte method first).
- Stability / developability ranking? Thermal shift assay.
- RNA (mRNA/circRNA)? IVT synthesis + qPCR.
General Ordering Workflow
- Select a protocol at https://cloud.ginkgo.bio/protocols
- Configure parameters (number of proteins/samples/molecules/targets, replicates, plates)
- Download the protocol's input template and upload inputs (FASTA/CSV/XLSX for sequence protocols; Design Tool for pixel art; vendor catalog numbers for onboarding)
- Add any special requirements in the Additional Details field
- Provide an email, agree to the protocol terms, and add to cart / submit to receive a feasibility report and price quote
For protocols not listed above, use the EstiMate chat (https://cloud.ginkgo.bio/estimate) to describe a custom protocol in plain language and receive a compatibility assessment and pricing.
Authentication
Access Ginkgo Cloud Lab at https://cloud.ginkgo.bio. Account creation or institutional access may be required. Contact Ginkgo at cloud@ginkgo.bio for access questions.
Key Infrastructure
- RACs (Reconfigurable Automation Carts): Modular robotic units with high-precision arms and maglev transport
- Catalyst Software: Protocol orchestration, scheduling, parameterization, and real-time monitoring
- 70+ integrated instruments: Agilent Bravo liquid handlers, Beckman/Labcyte Echo acoustic dispensers, BMG PHERAstar / Tecan Spark readers, Revvity LabChip, Bio-Rad CFX Opus, Nicoya Alto SPR, SciEx Echo-MS, Inheco/Cytomat incubators, and more
- Nebula: Ginkgo's autonomous lab facility in Boston, MA
1---2name: ginkgo-cloud-lab3description: Submit and manage protocols on Ginkgo Bioworks Cloud Lab (cloud.ginkgo.bio), a web-based interface for autonomous lab execution on Reconfigurable Automation Carts (RACs). Use when the user wants to run protein expression and purification (cell-free, E. coli, or Pichia), HiBiT or A280 or LabChip quantification, IVT mRNA/circRNA synthesis, thermal shift / developability assays, Echo-MS enzyme or analyte methods, SPR target onboarding, fluorescent pixel art, or otherwise interact with Ginkgo Cloud Lab services. Covers protocol selection, input preparation, pricing, and ordering workflows.4license: MIT license5---67# Ginkgo Cloud Lab89## Overview1011Ginkgo Cloud Lab (https://cloud.ginkgo.bio) provides remote access to Ginkgo Bioworks' autonomous lab infrastructure. Protocols are executed on Reconfigurable Automation Carts (RACs) -- modular units with robotic arms, maglev sample transport, and industrial-grade software spanning 70+ instruments.1213The platform also includes **EstiMate**, an AI agent that accepts human-language protocol descriptions and returns feasibility assessments and pricing for custom workflows beyond the listed protocols.1415The catalog is organized into **Expression & Purification** (in vitro / cell-free / E. coli / Pichia), **Characterization & Assay**, **Method & Target Onboarding**, and **Specialty**. Pick a protocol below, then read its reference file for inputs, outputs, the automated workflow, and ordering details.1617## Available Protocols1819### Expression & Purification - In vitro2021| Protocol | Readout | Price | Turnaround | Status |22|---|---|---|---|---|23| [IVT mRNA/circRNA Synthesis](references/ivt-rna-synthesis-qpcr.md) | qPCR (mRNA or circRNA, 384-well) | $99/sample | up to 12 business days | Certified |2425### Expression & Purification - Cell-free (E. coli CFPS)2627| Protocol | Readout | Price | Turnaround | Status |28|---|---|---|---|---|29| [Validate sequence expression](references/cell-free-protein-expression-validation.md) | Go/no-go titer + purity (up to 1800 bp) | $39/sample | up to 10 days | Certified |30| [Optimize expression conditions](references/cell-free-protein-expression-optimization.md) | DoE across 24 conditions | $199/sample | up to 11 days | Certified |31| [Express + quantify (HiBiT)](references/cell-free-protein-expression-hibit.md) | Luminescence, no purification | $39/sample | up to 11 days | Certified |32| [Express + purify (A280)](references/cfps-strep-tag-purification-a280.md) | Strep-tag, A280 yield | $149/sample | up to 11 days | Certified |33| [Express + purify minibinder](references/minibinder-strep-tag-a280.md) | Strep-tag, A280, LabChip | $149/sample | up to 11 days | Certified |34| [Express + purify (A280 + LabChip)](references/cfps-expression-purification-quantification.md) | Strep-tag, A280 + purity/size | $159/sample | up to 12 days | Certified |3536### Expression & Purification - E. coli3738| Protocol | Readout | Price | Turnaround | Status |39|---|---|---|---|---|40| [Express + quantify (HiBiT)](references/ecoli-protein-expression-hibit.md) | Luminescence (up to 384 constructs) | $79/sample | up to 3 weeks | Certified |41| [Express + purify (A280)](references/ecoli-protein-expression-histag-a280.md) | His-tag, A280 yield | $199/sample | up to 3 weeks | Certified |42| [Express + purify minibinder](references/ecoli-minibinder-expression-histag-a280.md) | His-tag, A280 yield | $199/sample | up to 3 weeks | Certified |43| [Express + purify (A280 + LabChip)](references/ecoli-expression-purification-quantification.md) | His-tag, A280 + purity/size | $209/sample | up to 3 weeks | Certified |4445### Expression & Purification - Pichia4647| Protocol | Readout | Price | Turnaround | Status |48|---|---|---|---|---|49| [Express + quantify (LabChip)](references/pichia-protein-expression-labchip.md) | Secreted protein, size/purity (up to 96) | $89/sample | up to 4 weeks | Certified (New) |5051### Characterization & Assay5253| Protocol | Readout | Price | Turnaround | Status |54|---|---|---|---|---|55| [Express + thermal shift](references/cfps-strep-purification-thermal-shift.md) | SYPRO Orange Tm (Tonset, TM1-3) | $159/sample | up to 12 days | Certified |56| [Detect enzymatic products (Echo-MS)](references/echo-ms-cfps-detection.md) | Substrate/product by Echo-MS | $44/sample | up to 13 days | Beta |5758### Method & Target Onboarding5960| Protocol | Readout | Price | Turnaround | Status |61|---|---|---|---|---|62| [Onboard Echo-MS method](references/echo-ms-method-onboarding.md) | Calibration curve, LOD/LOQ | $799/molecule | up to 3 weeks | Certified |63| [Onboard SPR target](references/spr-target-onboarding.md) | Validated SPR capture method | $1,399/target | up to 4 weeks | Beta |6465### Specialty6667| Protocol | Readout | Price | Turnaround | Status |68|---|---|---|---|---|69| [Generate fluorescent pixel art](references/fluorescent-pixel-art-generation.md) | UV photo, 7-color E. coli palette | $25/plate | up to 7 days | Beta |7071**Coming soon:** Protein Expression and Binding Affinity Characterization (express + purify, then screen binding affinity against a target).7273## Choosing a Protocol7475- **Quick expressibility screen?** Cell-free HiBiT ($39) or Validate sequence expression ($39).76- **Need purified protein + yield?** A280 tiers (cell-free or E. coli); add LabChip for purity/size.77- **Difficult / membrane / disulfide / cofactor targets?** Cell-free Optimize (24-condition DoE).78- **Secreted or eukaryotic targets?** Pichia expression.79- **Screening de novo binders/minibinders?** Cell-free or E. coli minibinder tiers, then SPR onboarding for kinetics.80- **Enzyme activity / biocatalysis?** Echo-MS enzymatic detection (onboard the analyte method first).81- **Stability / developability ranking?** Thermal shift assay.82- **RNA (mRNA/circRNA)?** IVT synthesis + qPCR.8384## General Ordering Workflow85861. Select a protocol at https://cloud.ginkgo.bio/protocols872. Configure parameters (number of proteins/samples/molecules/targets, replicates, plates)883. Download the protocol's input template and upload inputs (FASTA/CSV/XLSX for sequence protocols; Design Tool for pixel art; vendor catalog numbers for onboarding)894. Add any special requirements in the Additional Details field905. Provide an email, agree to the protocol terms, and add to cart / submit to receive a feasibility report and price quote9192For protocols not listed above, use the **EstiMate** chat (https://cloud.ginkgo.bio/estimate) to describe a custom protocol in plain language and receive a compatibility assessment and pricing.9394## Authentication9596Access Ginkgo Cloud Lab at https://cloud.ginkgo.bio. Account creation or institutional access may be required. Contact Ginkgo at cloud@ginkgo.bio for access questions.9798## Key Infrastructure99100- **RACs (Reconfigurable Automation Carts):** Modular robotic units with high-precision arms and maglev transport101- **Catalyst Software:** Protocol orchestration, scheduling, parameterization, and real-time monitoring102- **70+ integrated instruments:** Agilent Bravo liquid handlers, Beckman/Labcyte Echo acoustic dispensers, BMG PHERAstar / Tecan Spark readers, Revvity LabChip, Bio-Rad CFX Opus, Nicoya Alto SPR, SciEx Echo-MS, Inheco/Cytomat incubators, and more103- **Nebula:** Ginkgo's autonomous lab facility in Boston, MA
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