Visse

vissE

Workflows

Standard Workflow

Summarize and interpret a list of significant gene sets from an enrichment analysis using network clustering, text-mining, and gene/PPI visualization.

library(msigdb)
library(GSEABase)
library(vissE)
library(igraph)
library(ggplot2)
library(patchwork)

# 1. READ USER INPUT FILES (Simulated here)
msigdb_hs = getMsigdb()
msigdb_hs = subsetCollection(msigdb_hs, c('h', 'c2', 'c5'))
set.seed(360)
geneset_res = sample(sapply(msigdb_hs, setName), 2500)
geneset_gsc = msigdb_hs[geneset_res]

# 2. COMPUTE GENE-SET OVERLAP & NETWORK
gs_ovlap = computeMsigOverlap(geneset_gsc, thresh = 0.25)
gs_ovnet = computeMsigNetwork(gs_ovlap, msigdb_hs)

# 3. IDENTIFY CLUSTERS
geneset_stats = rnorm(2500)
names(geneset_stats) = geneset_res
grps = findMsigClusters(gs_ovnet, genesetStat = geneset_stats, alg = cluster_walktrap, minSize = 5)

# 4. CHARACTERIZE CLUSTERS (TEXT-MINING)
p1 = plotMsigWordcloud(msigdb_hs, grps[1:6], type = 'Name')

# 5. VISUALIZE GENE-LEVEL STATISTICS
genes = unique(unlist(geneIds(geneset_gsc)))
gene_stats = rnorm(length(genes))
names(gene_stats) = genes
p3 = plotGeneStats(gene_stats, msigdb_hs, grps[1:6]) + geom_hline(yintercept = 0, colour = 2, lty = 2)

# 6. VISUALIZE PROTEIN-PROTEIN INTERACTIONS
ppi = getIMEX('hs', inferred = TRUE)
p4 = plotMsigPPI(ppi, msigdb_hs, grps[1:6], geneStat = gene_stats, threshStatistic = 0.2, threshConfidence = 0.2)

# 7. COMBINE VISUALIZATIONS
p2 = plotMsigNetwork(gs_ovnet, markGroups = grps[1:6], genesetStat = geneset_stats)
p1 + p2 + p3 + p4 + plot_layout(2, 2)

Input: A GeneSetCollection object containing significant gene sets. Output: A combined paneled plot summarizing the gene-set clusters, text-mining word clouds, gene statistics, and protein-protein interactions.

When to Use

• To summarize large lists of significant gene sets from enrichment analyses (e.g., from limma::fry, singscore, or GSEA) using network-based clustering.
• To perform text-mining on gene-set names or short descriptions using frequency analysis (adjusted with inverse document frequency) to identify recurring biological themes.
• To visualize gene-level statistics (using plotGeneStats) and protein-protein interactions (using plotMsigPPI) within identified gene-set clusters.

When NOT to Use

• For performing the initial differential expression or gene-set enrichment calculation itself; use packages like limma or singscore first.
• When you do not have gene-set definitions or a GeneSetCollection object; vissE requires these to compute overlaps.

Data Requirements

• A GeneSetCollection object (e.g., from GSEABase or msigdb) representing the significant gene sets.
• A named numeric vector of gene-set statistics (e.g., p-values or FDRs) where names match the gene-set names.
• A named numeric vector of gene-level statistics (e.g., log fold-changes) where names match gene identifiers (e.g., Gene Symbols).

Key Parameters

• thresh (0.25): Threshold for Jaccard index or overlap coefficient in computeMsigOverlap.
• alg (cluster_walktrap): Graph clustering algorithm from igraph used in findMsigClusters.
• minSize (5): Minimum cluster size to retain in findMsigClusters.
• type ('Name'): Type of text-mining source ('Name' or 'Short') in plotMsigWordcloud.
• threshStatistic (0.2): Minimum gene-level statistic threshold for filtering nodes in plotMsigPPI.
• threshConfidence (0.2): Minimum confidence score for filtering PPI edges in plotMsigPPI.

Best Practices

• Filter MSigDB collections to recommended subsets (e.g., 'h', 'c2', 'c5') using subsetCollection to reduce noise.
• Set a random seed using set.seed before plotting networks (plotMsigNetwork) or PPIs to ensure reproducible layouts.
• Combine multiple visualization panels (word clouds, networks, gene stats, and PPIs) using patchwork (+ and plot_layout) for collective interpretation.

Common Pitfalls

• Unmatched gene identifiers: Ensure the gene IDs in your gene-level statistics vector match the ID type (e.g., Symbol vs Ensembl) used in the GeneSetCollection.
• Extremely dense networks: If the overlap network is too crowded, increase the thresh parameter in computeMsigOverlap to filter out weak overlaps.

Alternatives

• enrichplot for alternative visualization of enrichment results.
• clusterProfiler for functional profiling and gene set enrichment analysis.

Citations

• Bhuva DD (2026). vissE: Visualising Set Enrichment Analysis Results. R package.

References

• Homepage: bioconductor.org/packages/vissE
• Vignette: https://bioconductor.org/packages/release/bioc/vignettes/vissE/inst/doc/vissE.html