ffsshhttiikk What I do
- Design and construct new biological parts
- Engineer metabolic pathways
- Create synthetic gene circuits
- Develop biosensors and therapeutics
- Program cells with new functions
- Apply design-build-test-learn cycles
When to use me
- When engineering new biological functions
- When designing gene circuits
- When creating metabolic pathways
- When developing biosensors
- When programming cells
- When building synthetic organisms
Key Concepts
Design Principles
Standardization
- BioBricks: Standardized parts
- RFC standards: Assembly rules
- SBOL: Data exchange format
Genetic Circuits
# Example: Toggle switch design
class ToggleSwitch:
"""
Bistable genetic switch.
"""
def __init__(self):
self.components = {
'promoter1': 'Repressor 1',
'promoter2': 'Repressor 2',
'repressor1': 'Inhibits promoter2',
'repressor2': 'Inhibits promoter1'
}
def states(self):
return {
'state_A': 'Repressor1 ON, Repressor2 OFF',
'state_B': 'Repressor1 OFF, Repressor2 ON'
}
# Circuit types
circuit_types = {
'inverter': 'NOT gate',
'amplifier': 'Signal enhancement',
'oscillator': 'Repressible gene expression',
'memory': 'State persistence',
'logic_gates': 'AND, OR, NAND, NOR'
}
Metabolic Engineering
- Pathway design
- Cofactor balancing
- Flux optimization
- Enzyme engineering
- Compartmentalization
Applications
- Biofuels production
- Pharmaceutical synthesis
- Bioremediation
- Biosensors
- Therapeutic cells
- Living materials