name: bio-reporting-automated-qc-reports
description: Generates standardized quality control reports by aggregating metrics from FastQC, alignment, and other tools using MultiQC. Use when summarizing QC metrics across samples, creating shareable quality reports, or building automated QC pipelines.
tool_type: cli
primary_tool: multiqc
measurable_outcome: Execute skill workflow successfully with valid output within 15 minutes.
allowed-tools:
- read_file
- run_shell_command
Automated QC Reports with MultiQC
Basic Usage
# Aggregate all QC outputs in directory
multiqc results/ -o qc_report/
# Specify output name
multiqc results/ -n my_project_qc
# Include specific tools only
multiqc results/ --module fastqc --module star
Supported Tools
MultiQC recognizes outputs from 100+ bioinformatics tools:
Category
Tools
Read QC
FastQC, fastp, Cutadapt
Alignment
STAR, HISAT2, BWA, Bowtie2
Quantification
featureCounts, Salmon, kallisto
Variant Calling
bcftools, GATK
Single-cell
CellRanger, STARsolo
Configuration
Create multiqc_config.yaml:
title: "RNA-seq QC Report"
subtitle: "Project XYZ"
intro_text: "QC metrics for all samples"
# Custom sample name cleaning
extra_fn_clean_exts:
- '.sorted'
- '.dedup'
# Report sections to include
module_order:
- fastqc
- star
- featurecounts
# Highlight samples
table_cond_formatting_rules:
pct_mapped:
fail: [{lt: 50}]
warn: [{lt: 70}]
Run npx skillmds@latest add freedomintelligence/bio-reporting-automated-qc-reports in your terminal (requires Node.js), paste this page's agent-chat prompt into Claude, Cursor, or any MCP-connected agent, or download the SKILL.md file and copy it into your agent's skills directory.
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