Seed Representation Variant Comparison
Summary
Systematically evaluate whether alternative seed representations (sparse fixed-k anchors, reference k-mer variants, unitig-constrained uni-MEMs) produce stable mapping rates and per-transcript abundance quantification compared to a baseline seed strategy on identical reference indices. This skill detects whether seed granularity or representation choice explains discrepancies between mapper implementations.
When to use
You have observed mapping rate or quantification disagreement (e.g., >0.1% divergence in mapping rate or Pearson r < 0.999 in per-transcript counts) between two mappers or two versions of the same mapper on byte-identical indices, and you need to isolate whether the difference is due to seed representation strategy rather than indexing, orientation, or chain-pruning logic.
When NOT to use
- The two mappers or versions use different index formats or reference sequences (not byte-identical); index differences confound seed representation comparisons.
- You are comparing mappers with known differences in chain-pruning thresholds, alignment scoring, or poly-A clipping logic; these pre-seed-extraction filters will dominate quantification differences.
- Your input is already a quantification table (quant.sf or counts matrix); you need raw mapping output and per-read agreement, not downstream inference results.
Inputs
- Paired-end sequencing reads (FASTQ or gzip-compressed, e.g., 36.35M 76 bp reads)
- Byte-identical reference transcriptome index (salmon format or equivalent k-mer lookup structure)
- Seed representation configuration parameters (anchor density, k-mer length, MEM extraction strategy)
Outputs
- Mapping rate (%) per seed variant
- Per-read mapping agreement matrix (%) between variants
- Per-transcript NumReads quantification vectors (one per variant)
- Pearson correlation coefficient (r) matrix between NumReads across variants
- Summary table confirming r ≥ 0.99999995 for all pairwise comparisons
How to apply
Run the mapper in selective-alignment mode (e.g., salmon quant -l A -p <threads>) on a canonical paired-end read dataset (such as GEUVADIS ERR188044, 36.35M 76 bp reads) against a byte-identical reference index (e.g., GRCh38 cDNA, 193,759 transcripts). Execute the mapper at least four times, each with a different seed representation: (1) default/baseline (e.g., unitig-constrained approach), (2) sparse fixed-k anchors, (3) reference-extended MEM representation, and (4) true unitig-constrained uni-MEMs. Record per-read mapping agreement, overall mapping rate (%), and per-transcript NumReads output for each run. Calculate Pearson correlation coefficient between NumReads from each variant and the baseline. Accept the seed representation as stable if mapping rate remains within ≤0.01% of baseline and Pearson r for all variants meets or exceeds 0.99999995 threshold (demonstrating that seed representation granularity does not explain observed implementation differences).
Related tools
- salmon 2.0 (Primary mapper with configurable seed representations (default unitig-constrained, sparse fixed-k anchors, reference-extended MEMs) in selective-alignment mode) — https://github.com/COMBINE-lab/salmon
- salmon 1.12.0 (C++) (Baseline or reference implementation for cross-version seed representation validation) — https://github.com/COMBINE-lab/salmon/tree/cpp
- piscem-rs (Rust k-mer lookup and seed extraction engine underlying salmon 2.0, with configurable MEM and anchor strategies) — https://github.com/COMBINE-lab/salmon
- pufferfish (SSHash k-mer index lookup (critical for seed orientation and canonical k-mer retrieval; orientation correctness affects seed-based chain formation))
Examples
salmon quant -i salmon_index -l A -1 ERR188044_1.fastq.gz -2 ERR188044_2.fastq.gz -p 16 -o quant_baseline && salmon quant -i salmon_index -l A --seed-strategy sparse-anchor -1 ERR188044_1.fastq.gz -2 ERR188044_2.fastq.gz -p 16 -o quant_sparse_anchor && Rscript -e "baseline <- read.table('quant_baseline/quant.sf'); sparse <- read.table('quant_sparse_anchor/quant.sf'); cat('Pearson r =', cor(baseline[,4], sparse[,4]), '
')"
Evaluation signals
- Mapping rate remains within ≤0.01% of baseline across all seed variants (e.g., 85.55% ± 0.0085 for 36.35M reads)
- Per-read mapping agreement ≥99.83% between baseline and all seed variants, indicating reads map to identical or near-identical chain positions
- Pearson correlation (r) of per-transcript NumReads ≥0.99999995 between baseline and each variant (demonstrating high quantitative agreement despite seed granularity differences)
- Scatterplot of NumReads baseline vs. each variant shows tight clustering along y=x with no systematic offset or heteroscedasticity
- The union of reads mapped across all seed variants contains <0.1% more reads than the baseline alone (seed representation does not recover substantial new mappable regions)
Limitations
- Seed representation stability does not rule out downstream factors (chain pruning, orphan threshold, alignment scoring, poly-A clipping) that may still explain implementation differences; this skill isolates seed extraction alone.
- Pearson correlation threshold (0.99999995) assumes sufficient per-transcript read depth and coverage; transcripts with <1 NumRead may exhibit high correlation variance even if mappings are identical.
- The comparison assumes byte-identical reference indices; differences in k-mer precomputation, bloom-filter size, or decoy handling will confound results.
- Sparse fixed-k anchor and reference-extended MEM strategies may not be exposed as configuration flags in all mapper implementations; custom builds or source modifications may be required.
Evidence
- [other] Does the mapping rate and NumReads Pearson correlation remain stable when the Rust mapper uses alternative seed representations (sparse fixed-k anchors, reference k-mer variants) instead of the default unitig-constrained approach?: "Does the mapping rate and NumReads Pearson correlation remain stable when the Rust mapper uses alternative seed representations (sparse fixed-k anchors, reference k-mer variants) instead of the"
- [other] On byte-identical index, NumReads Pearson correlation between C++ and Rust is 0.99854, demonstrating high quantitative agreement across implementations and seed strategies.: "On byte-identical index, NumReads Pearson correlation between C++ and Rust is 0.99854, demonstrating high quantitative agreement across implementations and seed strategies."
- [other] Calculate Pearson correlation coefficient (r) between NumReads counts from each variant and the default baseline using correlation analysis, verifying r ≥ 0.99999995 for all variants.: "Calculate Pearson correlation coefficient (r) between NumReads counts from each variant and the default baseline using correlation analysis, verifying r ≥ 0.99999995 for all variants."
- [other] confirm mapping rate remains within ≤0.01% of baseline and Pearson r for all three variants meets or exceeds 0.99999995 threshold: "confirm mapping rate remains within ≤0.01% of baseline and Pearson r for all three variants meets or exceeds 0.99999995 threshold"
- [other] Run salmon 2.0 Rust mapper in selective-alignment mode with default seed representation (-l A, no bias correction, -p for multi-threading) and record mapping rate and per-transcript NumReads output.: "Run salmon 2.0 Rust mapper in selective-alignment mode with default seed representation (-l A, no bias correction, -p for multi-threading) and record mapping rate and per-transcript NumReads output."
- [other] Re-run salmon mapper with sparse fixed-k anchor representation, capturing mapping rate and NumReads correlation (Pearson r) against baseline.: "Re-run salmon mapper with sparse fixed-k anchor representation, capturing mapping rate and NumReads correlation (Pearson r) against baseline."
- [other] seed representation granularity does not explain prior C++ vs. Rust mapping differences: "establishing that seed representation granularity does not explain prior C++ vs. Rust mapping differences"
- [readme] It keeps the same workflow (
salmon index → salmon quant → quant.sf) and the same output formats downstream tools read: "It keeps the same workflow (salmon index → salmon quant → quant.sf) and the same output formats downstream tools read"
1---2name: seed-representation-variant-comparison3description: Use when you have observed mapping rate or quantification disagreement (e.g., >0.1% divergence in mapping rate or Pearson r < 0.4license: CC-BY-4.05---67# Seed Representation Variant Comparison89## Summary1011Systematically evaluate whether alternative seed representations (sparse fixed-k anchors, reference k-mer variants, unitig-constrained uni-MEMs) produce stable mapping rates and per-transcript abundance quantification compared to a baseline seed strategy on identical reference indices. This skill detects whether seed granularity or representation choice explains discrepancies between mapper implementations.1213## When to use1415You have observed mapping rate or quantification disagreement (e.g., >0.1% divergence in mapping rate or Pearson r < 0.999 in per-transcript counts) between two mappers or two versions of the same mapper on byte-identical indices, and you need to isolate whether the difference is due to seed representation strategy rather than indexing, orientation, or chain-pruning logic.1617## When NOT to use1819- The two mappers or versions use different index formats or reference sequences (not byte-identical); index differences confound seed representation comparisons.20- You are comparing mappers with known differences in chain-pruning thresholds, alignment scoring, or poly-A clipping logic; these pre-seed-extraction filters will dominate quantification differences.21- Your input is already a quantification table (quant.sf or counts matrix); you need raw mapping output and per-read agreement, not downstream inference results.2223## Inputs2425- Paired-end sequencing reads (FASTQ or gzip-compressed, e.g., 36.35M 76 bp reads)26- Byte-identical reference transcriptome index (salmon format or equivalent k-mer lookup structure)27- Seed representation configuration parameters (anchor density, k-mer length, MEM extraction strategy)2829## Outputs3031- Mapping rate (%) per seed variant32- Per-read mapping agreement matrix (%) between variants33- Per-transcript NumReads quantification vectors (one per variant)34- Pearson correlation coefficient (r) matrix between NumReads across variants35- Summary table confirming r ≥ 0.99999995 for all pairwise comparisons3637## How to apply3839Run the mapper in selective-alignment mode (e.g., `salmon quant -l A -p <threads>`) on a canonical paired-end read dataset (such as GEUVADIS ERR188044, 36.35M 76 bp reads) against a byte-identical reference index (e.g., GRCh38 cDNA, 193,759 transcripts). Execute the mapper at least four times, each with a different seed representation: (1) default/baseline (e.g., unitig-constrained approach), (2) sparse fixed-k anchors, (3) reference-extended MEM representation, and (4) true unitig-constrained uni-MEMs. Record per-read mapping agreement, overall mapping rate (%), and per-transcript NumReads output for each run. Calculate Pearson correlation coefficient between NumReads from each variant and the baseline. Accept the seed representation as stable if mapping rate remains within ≤0.01% of baseline and Pearson r for all variants meets or exceeds 0.99999995 threshold (demonstrating that seed representation granularity does not explain observed implementation differences).4041## Related tools4243- **salmon 2.0** (Primary mapper with configurable seed representations (default unitig-constrained, sparse fixed-k anchors, reference-extended MEMs) in selective-alignment mode) — https://github.com/COMBINE-lab/salmon44- **salmon 1.12.0 (C++)** (Baseline or reference implementation for cross-version seed representation validation) — https://github.com/COMBINE-lab/salmon/tree/cpp45- **piscem-rs** (Rust k-mer lookup and seed extraction engine underlying salmon 2.0, with configurable MEM and anchor strategies) — https://github.com/COMBINE-lab/salmon46- **pufferfish** (SSHash k-mer index lookup (critical for seed orientation and canonical k-mer retrieval; orientation correctness affects seed-based chain formation))4748## Examples4950```51salmon quant -i salmon_index -l A -1 ERR188044_1.fastq.gz -2 ERR188044_2.fastq.gz -p 16 -o quant_baseline && salmon quant -i salmon_index -l A --seed-strategy sparse-anchor -1 ERR188044_1.fastq.gz -2 ERR188044_2.fastq.gz -p 16 -o quant_sparse_anchor && Rscript -e "baseline <- read.table('quant_baseline/quant.sf'); sparse <- read.table('quant_sparse_anchor/quant.sf'); cat('Pearson r =', cor(baseline[,4], sparse[,4]), '52')"53```5455## Evaluation signals5657- Mapping rate remains within ≤0.01% of baseline across all seed variants (e.g., 85.55% ± 0.0085 for 36.35M reads)58- Per-read mapping agreement ≥99.83% between baseline and all seed variants, indicating reads map to identical or near-identical chain positions59- Pearson correlation (r) of per-transcript NumReads ≥0.99999995 between baseline and each variant (demonstrating high quantitative agreement despite seed granularity differences)60- Scatterplot of NumReads baseline vs. each variant shows tight clustering along y=x with no systematic offset or heteroscedasticity61- The union of reads mapped across all seed variants contains <0.1% more reads than the baseline alone (seed representation does not recover substantial new mappable regions)6263## Limitations6465- Seed representation stability does not rule out downstream factors (chain pruning, orphan threshold, alignment scoring, poly-A clipping) that may still explain implementation differences; this skill isolates seed extraction alone.66- Pearson correlation threshold (0.99999995) assumes sufficient per-transcript read depth and coverage; transcripts with <1 NumRead may exhibit high correlation variance even if mappings are identical.67- The comparison assumes byte-identical reference indices; differences in k-mer precomputation, bloom-filter size, or decoy handling will confound results.68- Sparse fixed-k anchor and reference-extended MEM strategies may not be exposed as configuration flags in all mapper implementations; custom builds or source modifications may be required.6970## Evidence7172- [other] Does the mapping rate and NumReads Pearson correlation remain stable when the Rust mapper uses alternative seed representations (sparse fixed-k anchors, reference k-mer variants) instead of the default unitig-constrained approach?: "Does the mapping rate and NumReads Pearson correlation remain stable when the Rust mapper uses alternative seed representations (sparse fixed-k anchors, reference k-mer variants) instead of the"73- [other] On byte-identical index, NumReads Pearson correlation between C++ and Rust is 0.99854, demonstrating high quantitative agreement across implementations and seed strategies.: "On byte-identical index, NumReads Pearson correlation between C++ and Rust is 0.99854, demonstrating high quantitative agreement across implementations and seed strategies."74- [other] Calculate Pearson correlation coefficient (r) between NumReads counts from each variant and the default baseline using correlation analysis, verifying r ≥ 0.99999995 for all variants.: "Calculate Pearson correlation coefficient (r) between NumReads counts from each variant and the default baseline using correlation analysis, verifying r ≥ 0.99999995 for all variants."75- [other] confirm mapping rate remains within ≤0.01% of baseline and Pearson r for all three variants meets or exceeds 0.99999995 threshold: "confirm mapping rate remains within ≤0.01% of baseline and Pearson r for all three variants meets or exceeds 0.99999995 threshold"76- [other] Run salmon 2.0 Rust mapper in selective-alignment mode with default seed representation (-l A, no bias correction, -p for multi-threading) and record mapping rate and per-transcript NumReads output.: "Run salmon 2.0 Rust mapper in selective-alignment mode with default seed representation (-l A, no bias correction, -p for multi-threading) and record mapping rate and per-transcript NumReads output."77- [other] Re-run salmon mapper with sparse fixed-k anchor representation, capturing mapping rate and NumReads correlation (Pearson r) against baseline.: "Re-run salmon mapper with sparse fixed-k anchor representation, capturing mapping rate and NumReads correlation (Pearson r) against baseline."78- [other] seed representation granularity does not explain prior C++ vs. Rust mapping differences: "establishing that seed representation granularity does not explain prior C++ vs. Rust mapping differences"79- [readme] It keeps the same workflow (`salmon index` → `salmon quant` → `quant.sf`) and the same output formats downstream tools read: "It keeps the same workflow (`salmon index` → `salmon quant` → `quant.sf`) and the same output formats downstream tools read"
HolobiomicsLab