Mutation Impact Analysis

Mutation Impact Analysis

Discipline: Molecular Biology | Tools Used: 4 | Servers: 3

Description

Analyze mutation impact: predict structure, predict mutations from sequence and structure, and check variant effects with Ensembl VEP.

Tools Used

• pred_protein_structure_esmfold from server-3 (streamable-http) - https://scp.intern-ai.org.cn/api/v1/mcp/3/DrugSDA-Model
• zero_shot_sequence_prediction from server-1 (sse) - https://scp.intern-ai.org.cn/api/v1/mcp/1/VenusFactory
• predict_zero_shot_structure from server-1 (sse) - https://scp.intern-ai.org.cn/api/v1/mcp/1/VenusFactory
• get_vep_hgvs from ensembl-server (streamable-http) - https://scp.intern-ai.org.cn/api/v1/mcp/12/Origene-Ensembl

Workflow

1. Predict protein structure
2. Predict mutations from sequence
3. Predict mutations from structure
4. Check variant effects with VEP

Test Case

Input

{
    "sequence": "MKTIIALSYIFCLVFA",
    "hgvs": "ENSP00000269305.4:p.Val600Glu"
}

Expected Steps

1. Predict protein structure
2. Predict mutations from sequence
3. Predict mutations from structure
4. Check variant effects with VEP

Usage Example

Note: Replace <YOUR_SCP_HUB_API_KEY> with your own SCP Hub API Key. You can obtain one from the SCP Platform.

import asyncio
import json
from mcp import ClientSession
from mcp.client.streamable_http import streamablehttp_client
from mcp.client.sse import sse_client

SERVERS = {
    "server-3": "https://scp.intern-ai.org.cn/api/v1/mcp/3/DrugSDA-Model",
    "server-1": "https://scp.intern-ai.org.cn/api/v1/mcp/1/VenusFactory",
    "ensembl-server": "https://scp.intern-ai.org.cn/api/v1/mcp/12/Origene-Ensembl"
}

async def connect(url, transport_type):
    transport = streamablehttp_client(url=url, headers={"SCP-HUB-API-KEY": "<YOUR_SCP_HUB_API_KEY>"})
    read, write, _ = await transport.__aenter__()
    ctx = ClientSession(read, write)
    session = await ctx.__aenter__()
    await session.initialize()
    return session, ctx, transport

def parse(result):
    try:
        if hasattr(result, 'content') and result.content:
            c = result.content[0]
            if hasattr(c, 'text'):
                try: return json.loads(c.text)
                except: return c.text
        return str(result)
    except: return str(result)

async def main():
    # Connect to required servers
    sessions = {}
    sessions["server-3"], _, _ = await connect("https://scp.intern-ai.org.cn/api/v1/mcp/3/DrugSDA-Model", "streamable-http")
    sessions["server-1"], _, _ = await connect("https://scp.intern-ai.org.cn/api/v1/mcp/1/VenusFactory", "sse")
    sessions["ensembl-server"], _, _ = await connect("https://scp.intern-ai.org.cn/api/v1/mcp/12/Origene-Ensembl", "streamable-http")

    # Execute workflow steps
    # Step 1: Predict protein structure
    result_1 = await sessions["server-3"].call_tool("pred_protein_structure_esmfold", arguments={})
    data_1 = parse(result_1)
    print(f"Step 1 result: {json.dumps(data_1, indent=2, ensure_ascii=False)[:500]}")

    # Step 2: Predict mutations from sequence
    result_2 = await sessions["server-1"].call_tool("zero_shot_sequence_prediction", arguments={})
    data_2 = parse(result_2)
    print(f"Step 2 result: {json.dumps(data_2, indent=2, ensure_ascii=False)[:500]}")

    # Step 3: Predict mutations from structure
    result_3 = await sessions["server-1"].call_tool("predict_zero_shot_structure", arguments={})
    data_3 = parse(result_3)
    print(f"Step 3 result: {json.dumps(data_3, indent=2, ensure_ascii=False)[:500]}")

    # Step 4: Check variant effects with VEP
    result_4 = await sessions["ensembl-server"].call_tool("get_vep_hgvs", arguments={})
    data_4 = parse(result_4)
    print(f"Step 4 result: {json.dumps(data_4, indent=2, ensure_ascii=False)[:500]}")

    # Cleanup
    print("Workflow complete!")

if __name__ == "__main__":
    asyncio.run(main())