vamseeachanta Calculated Data Handling
Calculated Data Handling
IMPORTANT: Separate raw measurements from calculated/derived values.
- Raw data ->
measurement-document(direct instrument readings) - Calculated data ->
calculated-data-aggregate-document(derived values)
Calculated values MUST include traceability via data-source-aggregate-document:
"calculated-data-aggregate-document": {
"calculated-data-document": [{
"calculated-data-identifier": "SAMPLE_B1_DIN_001",
"calculated-data-name": "DNA integrity number",
"calculated-result": {"value": 9.5, "unit": "(unitless)"},
"data-source-aggregate-document": {
"data-source-document": [{
"data-source-identifier": "SAMPLE_B1_MEASUREMENT",
"data-source-feature": "electrophoresis trace"
}]
}
}]
}
Common calculated fields by instrument type:
| Instrument | Calculated Fields |
|---|---|
| Cell counter | Viability %, cell density dilution-adjusted values |
| Spectrophotometer | Concentration (from absorbance), 260/280 ratio |
| Plate reader | Concentrations from standard curve, %CV |
| Electrophoresis | DIN/RIN, region concentrations, average sizes |
| qPCR | Relative quantities, fold change |
See references/field_classification_guide.md for detailed guidance on raw vs. calculated classification.