gabrielmoreira
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- ▌ Shopify Observability · gabrielmoreira bundleSet up observability for Shopify app integrations with query cost tracking, rate limit monitoring, webhook delivery metrics, and structured logging. Use when instrumenting a Shopify app for production monitoring, setting up Prometheus metrics for API health, or configuring alerts for rate limit issues. Trigger with phrases like "shopify monitoring", "shopify metrics", "shopify observability", "monitor shopify API", "shopify alerts", "shopify dashboard".
- ▌ Speak Core Workflow A · gabrielmoreira bundleExecute Speak primary workflow: AI Conversation Practice with real-time feedback. Use when implementing conversation practice features, building AI tutor interactions, or core language learning dialogue systems. Trigger with phrases like "speak conversation practice", "speak AI tutor", "speak dialogue", "primary speak workflow".
- ▌ Speak Core Workflow B · gabrielmoreira bundleExecute Speak secondary workflow: Pronunciation Training with phoneme-level analysis. Use when implementing pronunciation drills, speech scoring, or targeted pronunciation improvement features. Trigger with phrases like "speak pronunciation training", "speak speech scoring", "speak phoneme analysis".
- ▌ Speak Enterprise Rbac · gabrielmoreira bundleConfigure Speak for schools and organizations: SSO, teacher/student roles, class management, and usage reporting. Use when implementing enterprise rbac, or managing Speak language learning platform operations. Trigger with phrases like "speak enterprise rbac", "speak enterprise rbac".
- ▌ Speak Multi Env Setup · gabrielmoreira bundleConfigure Speak across dev, staging, and production with separate API keys and mock modes. Use when implementing multi env setup, or managing Speak language learning platform operations. Trigger with phrases like "speak multi env setup", "speak multi env setup".
- ▌ Speak Security Basics · gabrielmoreira bundleSecurity best practices for Speak API keys, audio data privacy, student data protection, and COPPA/FERPA compliance. Use when implementing security basics features, or troubleshooting Speak language learning integration issues. Trigger with phrases like "speak security basics", "speak security basics".
- ▌ Speak Webhooks Events · gabrielmoreira bundleHandle Speak lesson completion events, progress webhooks, and achievement notifications. Use when implementing webhooks events, or managing Speak language learning platform operations. Trigger with phrases like "speak webhooks events", "speak webhooks events".
- ▌ Supabase Debug Bundle · gabrielmoreira bundleCollect Supabase diagnostic info for troubleshooting and support tickets. Use when debugging connection failures, auth issues, Realtime drops, Storage errors, RLS misconfigurations, or preparing a support escalation. Trigger: "supabase debug", "supabase diagnostics", "supabase support bundle", "collect supabase logs", "debug supabase connection".
- ▌ Supabase Install Auth · gabrielmoreira bundleInstall and configure Supabase SDK, CLI, and project authentication. Use when setting up a new Supabase project, installing @supabase/supabase-js, configuring environment variables, or initializing the Supabase client. Trigger with "install supabase", "setup supabase", "supabase auth config", "configure supabase", "supabase init", "add supabase to project".
- ▌ Supabase Sdk Patterns · gabrielmoreira bundleUse when implementing Supabase queries, auth, realtime, storage, or RPC calls with @supabase/supabase-js or supabase-py and you need production-ready, type-safe patterns that always check the { data, error } contract. Trigger with phrases like "supabase SDK patterns", "supabase query", "supabase typescript", "supabase python", "supabase client setup", "supabase realtime", "supabase auth", "supabase storage".
- ▌ Techsmith Hello World · gabrielmoreiraCapture a screenshot with Snagit COM API and produce a Camtasia video. Use when automating screen captures, batch-processing recordings, or building documentation pipelines with TechSmith tools. Trigger: "techsmith hello world, snagit capture, camtasia render".
- ▌ Together Install Auth · gabrielmoreiraInstall Together AI SDK and configure API key for inference and fine-tuning. Use when setting up Together AI, configuring the OpenAI-compatible API, or initializing the together Python package. Trigger: "install together, setup together ai, together API key".
- ▌ Twinmind Debug Bundle · gabrielmoreiraCollect comprehensive diagnostic information for TwinMind issues. Use when preparing support requests, investigating complex problems, or gathering evidence for bug reports. Trigger with phrases like "twinmind debug", "twinmind diagnostics", "collect twinmind info", "twinmind support bundle".
- ▌ Twinmind Install Auth · gabrielmoreiraInstall and configure TwinMind Chrome extension, mobile app, and API access. Use when setting up TwinMind for meeting transcription, configuring calendar integration, or initializing TwinMind in your workflow. Trigger with phrases like "install twinmind", "setup twinmind", "twinmind auth", "configure twinmind", "twinmind chrome extension".
- ▌ Twinmind Sdk Patterns · gabrielmoreiraApply production-ready TwinMind SDK patterns for TypeScript and Python. Use when implementing TwinMind integrations, refactoring API usage, or establishing team coding standards for meeting AI integration. Trigger with phrases like "twinmind SDK patterns", "twinmind best practices", "twinmind code patterns", "idiomatic twinmind".
- ▌ Vastai CI Integration · gabrielmoreiraConfigure Vast.ai CI/CD integration with GitHub Actions and automated GPU testing. Use when setting up automated testing on GPU instances, or integrating Vast.ai provisioning into CI/CD pipelines. Trigger with phrases like "vastai CI", "vastai github actions", "vastai automated testing", "vastai pipeline".
- ▌ Vastai Local Dev Loop · gabrielmoreiraConfigure Vast.ai local development with testing and fast iteration. Use when setting up a development environment, testing instance provisioning, or building a fast iteration cycle for GPU workloads. Trigger with phrases like "vastai dev setup", "vastai local development", "vastai dev environment", "develop with vastai".
- ▌ Vastai Prod Checklist · gabrielmoreiraExecute Vast.ai production deployment checklist for GPU workloads. Use when deploying training pipelines to production, preparing for large-scale GPU jobs, or auditing production readiness. Trigger with phrases like "vastai production", "deploy vastai", "vastai go-live", "vastai launch checklist".
- ▌ Vercel CI Integration · gabrielmoreira bundleConfigure Vercel CI/CD with GitHub Actions, preview deployments, and automated testing. Use when setting up automated deployments, configuring preview bots, or integrating Vercel into your CI pipeline. Trigger with phrases like "vercel CI", "vercel GitHub Actions", "vercel automated deploy", "CI vercel", "vercel pipeline".
- ▌ Vercel Deploy Preview · gabrielmoreiraCreate and manage Vercel preview deployments for branches and pull requests. Use when deploying a preview for a pull request, testing changes before production, or sharing preview URLs with stakeholders. Trigger with phrases like "vercel deploy preview", "vercel preview URL", "create preview deployment", "vercel PR preview".
- ▌ Vercel Known Pitfalls · gabrielmoreira bundleIdentify and avoid Vercel anti-patterns and common integration mistakes. Use when reviewing Vercel code for issues, onboarding new developers, or auditing existing Vercel deployments for best practice violations. Trigger with phrases like "vercel mistakes", "vercel anti-patterns", "vercel pitfalls", "vercel what not to do", "vercel code review".
- ▌ Vercel Local Dev Loop · gabrielmoreira bundleConfigure Vercel local development with vercel dev, environment variables, and hot reload. Use when setting up a development environment, testing serverless functions locally, or establishing a fast iteration cycle with Vercel. Trigger with phrases like "vercel dev setup", "vercel local development", "vercel dev environment", "develop with vercel locally".
- ▌ Vercel Prod Checklist · gabrielmoreira bundleVercel production deployment checklist with rollback and promotion procedures. Use when deploying to production, preparing for launch, or implementing go-live and instant rollback procedures. Trigger with phrases like "vercel production", "deploy vercel prod", "vercel go-live", "vercel launch checklist", "vercel promote".
- ▌ Webflow Common Errors · gabrielmoreiraDiagnose and fix Webflow Data API v2 errors — 400, 401, 403, 404, 409, 429, 500. Use when encountering Webflow API errors, debugging failed requests, or troubleshooting integration issues. Trigger with phrases like "webflow error", "fix webflow", "webflow not working", "debug webflow", "webflow 429", "webflow 401".
- ▌ Windsurf Code Privacy · gabrielmoreira bundleConfigure code privacy and data retention policies. Activate when users mention "code privacy", "data retention", "privacy settings", "data governance", or "gdpr compliance". Handles privacy and data protection configuration. Use when working with windsurf code privacy functionality. Trigger with phrases like "windsurf code privacy", "windsurf privacy", "windsurf".
- ▌ Windsurf Debug Bundle · gabrielmoreiraCollect Windsurf diagnostic information for troubleshooting and support tickets. Use when encountering persistent issues, preparing support tickets, or collecting diagnostic data for Windsurf problems. Trigger with phrases like "windsurf debug", "windsurf support", "windsurf diagnostic", "windsurf logs", "windsurf not working".
- ▌ Windsurf Debugging AI · gabrielmoreira bundleExecute use Cascade for intelligent debugging and error analysis. Activate when users mention "debug with ai", "error analysis", "cascade debug", "find bug", or "troubleshoot code". Handles AI-assisted debugging workflows. Use when debugging issues or troubleshooting. Trigger with phrases like "windsurf debugging ai", "windsurf ai", "windsurf".
- ▌ Windsurf Install Auth · gabrielmoreiraInstall Windsurf IDE and configure Codeium authentication. Use when setting up Windsurf for the first time, logging in to Codeium, or configuring API keys for team/enterprise deployments. Trigger with phrases like "install windsurf", "setup windsurf", "windsurf auth", "codeium login", "windsurf API key".
- ▌ Windsurf Sdk Patterns · gabrielmoreiraApply production-ready Windsurf workspace configuration and Cascade interaction patterns. Use when configuring .windsurfrules, workspace rules, MCP servers, or establishing team coding standards for Windsurf AI. Trigger with phrases like "windsurf patterns", "windsurf best practices", "windsurf config patterns", "windsurfrules", "windsurf workspace".
- ▌ Workhuman Cost Tuning · gabrielmoreiraWorkhuman cost tuning for employee recognition and rewards API. Use when integrating Workhuman Social Recognition, or building recognition workflows with HRIS systems. Trigger: "workhuman cost tuning".
- ▌ Workhuman Hello World · gabrielmoreiraWorkhuman hello world for employee recognition and rewards API. Use when integrating Workhuman Social Recognition, or building recognition workflows with HRIS systems. Trigger: "workhuman hello world".
- ▌ Sns Topic Config · gabrielmoreiraConfigure sns topic config operations. Auto-activating skill for AWS Skills. Triggers on: sns topic config, sns topic config Part of the AWS Skills skill category. Use when configuring systems or services. Trigger with phrases like "sns topic config", "sns config", "sns".
- ▌ Plan Cartonization · gabrielmoreira bundlePlan cartonization from item dimensions, weights, order mix, carton set, packing rules, and shipment constraints.
- ▌ Run Gauss · gabrielmoreiraActs as a knowledge base providing environment checklists, directory/scratch management, and bash command templates. USE WHEN you need to guide the execution of Gaussian computational chemistry jobs (.gjf) on local or remote/HPC environments.
- ▌ Perspectival Constellation · gabrielmoreiraStructure multi-POV stories through catalyst environments. Use when building interconnected narratives, when perspectives need meaningful intersection, or when a shared setting needs to generate distinct storylines.
- ▌ Systemic Worldbuilding · gabrielmoreiraBuild worlds through cascading consequences from speculative changes. Use when introducing new technologies, species, or alternate histories and need to trace realistic societal transformations across multiple domains.
- ▌ Analyse Dpa Fournisseur Hugo Salard · gabrielmoreira bundleAnalyse systématique d'un Data Processing Agreement (DPA) au regard de l'article 28 RGPD, des lignes directrices EDPB 07/2020 et 02/2024, des CCT 2021 (décision d'exécution 2021/914), des recommandations EDPB 01/2020 (mesures supplémentaires post-Schrems II), et du Règlement (UE) 2024/1689 (Règlement IA). Produit un rapport structuré clause par clause (18 clauses : 13 obligatoires + 5 complémentaires) avec diagnostic 🟢/🟡/🔴, remédiations prêtes à insérer, analyse détaillée des transferts internationaux, vérification Règlement IA, et questions à poser au fournisseur. Triggers : "analyse de DPA", "audit DPA", "vérifier un DPA", "DPA fournisseur", "data processing agreement", "art. 28 RGPD", "sous-traitant RGPD", "négociation DPA", "review DPA", "conformité contrat sous-traitance".
- ▌ Legal Translation · gabrielmoreira bundleTranslate legal documents from any language into English while preserving .docx formatting and translating track changes and headers/footers. Use this skill whenever the user asks to translate a legal document, contract, deed, agreement, or any formal legal text into English. Also trigger when the user mentions translating .docx files that contain legal content, or asks for a "format-preserving translation" of a legal document. This skill covers all legal domains: finance, M&A, corporate, IP, real estate, regulatory, consumer, taxes, litigation, SaaS and more. Even if the user just says "translate this contract" or "translate this into English", use this skill if the document is legal in nature.
- ▌ Knowledge Distillation · gabrielmoreiraCompile one or more OpenViking knowledge bases or document collections into topic-organized, evidence-grounded high-level knowledge, including cross-source findings, trends, changes, drivers, comparisons, implications, and uncertainties. Use with ov compile when the user asks to distill or synthesize a knowledge base, compare multiple collections, or derive higher-order insights such as changes across financial reports; do not use for document-by-document summaries.
- ▌ Install Openviking Memory · gabrielmoreiraInstall and configure the OpenViking long-term memory plugin for OpenClaw via natural conversation. Once installed, the plugin automatically captures facts from chats and recalls relevant context before each reply (auto-capture + auto-recall, cross-session). Covers prerequisites, install through OpenClaw's plugin manager (ov-install as backup), wizard-based configuration, slot activation, gateway restart, verification, multi-tenant root keys, multi-instance and uninstall. The user needs no CLI knowledge — the agent runs everything and only asks for a few values. Assumes the OpenViking server is already running; it does NOT install the server. Trigger on: "install OpenViking", "set up memory", "configure memory plugin", "add long-term memory", "semantic memory", "RAG", "帮我装 OpenViking", "配置记忆插件", "安装记忆功能", "接入 OpenViking".
- ▌ Raise Pull Requests · gabrielmoreiraRaise high-quality pull requests that are easy to review, provide clear context, and follow team conventions. Use when a user asks to open, write, prepare, or submit a pull request. Covers PR sizing, self-review, writing descriptions, submitting PRs, responding to review feedback, and reviewing others' PRs. Follows GitHub best practices for collaboration and code review.
- ▌ Openapi Spec Generation · gabrielmoreiraGenerate and maintain OpenAPI 3.1 specifications from code, design-first specs, and validation patterns. Use when creating API documentation, generating SDKs, or ensuring API contract compliance.
- ▌ Modern Javascript Patterns · gabrielmoreiraMaster ES6+ features including async/await, destructuring, spread operators, arrow functions, promises, modules, iterators, generators, and functional programming patterns for writing clean, efficient JavaScript code. Use when refactoring legacy code, implementing modern patterns, or optimizing JavaScript applications.
- ▌ Hybrid Search Implementation · gabrielmoreiraCombine vector and keyword search for improved retrieval. Use when implementing RAG systems, building search engines, or when neither approach alone provides sufficient recall.
- ▌ Meta Harness Proteus · gabrielmoreiraRun one iteration of proteus memory-summary evolution. Called by meta_harness.py.
- ▌ Message System Architect · gabrielmoreiraUse when the user asks to "author our durable brand message hierarchy", "build the brand message house that seeds the canon", or "define the main narrative, three pillars, and tagline for the whole brand"; produces the DURABLE brand message system — main narrative, three value pillars, per-persona proof points (each labeled Measured / User-provided / '[needs source]'), and the tagline/one-liner — that seeds the narrative-registry canon and from which every per-launch house derives. Not for a single launch's message house or PR-FAQ — use message-house-builder; not for claim adjudication — use offer-claims-registry; not for the change-narrative arc itself — use strategic-narrative-designer. 品牌消息屋/主叙事/三支柱/标语/持久叙事 canon
- ▌ Narrative Quality Auditor · gabrielmoreiraUse when the user asks to "audit our brand narrative" or "is this message on-canon"; runs separate typed TALE truth, system, or effectiveness profiles and never averages them into one composite. Checks differentiation, canon, landing consistency, and evidence integrity. Not for launch readiness — use launch-readiness-auditor; not for social operations — use social-quality-auditor. 品牌叙事分层审计/发布前一致性放行
- ▌ Method Writing · gabrielmoreiraWrite and revise the Methods section of research papers to ensure reproducibility; use when preparing an IMRAD manuscript or responding to journal/reporting-guideline requirements (e.g., CONSORT/STROBE/PRISMA).
- ▌ Singlecell Portal · gabrielmoreiraProgrammatically query public single-cell study metadata from the Broad Institute Single Cell Portal REST API when you need to search and filter datasets by organism, tissue, disease, or cell type without an API key.
- ▌ Torchdrug English · gabrielmoreiraPyTorch-native Graph Neural Network framework for molecules and proteins. Suitable for building custom GNN architectures for drug discovery, protein modeling, or knowledge graph reasoning. Best for custom model development, protein property prediction, and retrosynthesis. If you need pretrained models and diverse feature extractors, use deepchem; if you need benchmark datasets, use pytdc.
- ▌ Arxiv Database · gabrielmoreiraSearch and retrieve scientific preprints from arXiv; use it when you need to find papers by keyword/author/category, fetch metadata (abstract, DOI, PDF URL), or download PDFs for offline reading.
- ▌ Journal Skills · gabrielmoreiraRecommends target journals for manuscript submission by analyzing the paper topic/abstract and the journal distribution of similar PubMed literature; use when users ask for journal recommendation/matching, submission strategy, PubMed search, or similar-literature statistics.
- ▌ Reactome Skill · gabrielmoreiraQuery the Reactome REST API for pathway content and enrichment analyses; use when you need curated pathway data, reaction details, or overrepresentation results for a gene list.
- ▌ Scite Database · gabrielmoreiraAccess Scite.ai Smart Citations to classify how a paper is cited (supporting, contrasting, mentioning) and assess scientific claims; use it when you need to evaluate a paper’s reliability or its acceptance in the literature.
- ▌ Academic Poster Generator · gabrielmoreiraComplete workflow for generating academic research posters from PDF literature; use when you need to extract paper content from PDFs and produce a LaTeX-based poster (beamerposter/tikzposter/baposter) with mandatory figure generation and a final rendered HTML deliverable.
- ▌ Article Format Adjustment · gabrielmoreiraAdjust academic paper formatting and convert between DOCX/LaTeX/Markdown when you need to meet a journal or school template requirement.
- ▌ Equipment Maintenance Log · gabrielmoreiraTrack lab equipment calibration dates and send maintenance reminders for pipettes, balances, centrifuges, and other instruments. Validates date formats and supports update/delete operations.
- ▌ Lab Result Interpretation · gabrielmoreiraTransforms biochemical lab test results into clear, patient-friendly explanations. Covers blood routine, lipid panel, liver/kidney function, thyroid, electrolytes, and inflammation markers. Flags critical values, classifies severity, and generates structured interpretation rep...
- ▌ Medication Reconciliation · gabrielmoreiraCompare patient pre-admission medication lists with inpatient orders to automatically identify omitted or duplicated medications and improve medication safety.
- ▌ Meeting Minutes Generator · gabrielmoreiraGenerates structured meeting minutes from text transcripts. Use when the user provides text content and wants a structured summary with a signature.
- ▌ Shift Handover Summarizer · gabrielmoreiraGenerate structured shift handover summaries from EHR records, highlighting critical events, vital sign changes, and pending tasks for incoming clinical staff.
- ▌ Text To Technical Roadmap · gabrielmoreiraConverts research text into a Mermaid technical roadmap flowchart. Use when the user provides research proposals, experiment designs, or scientific text and asks for a roadmap or flowchart.
- ▌ Cinematic Web Experiences · gabrielmoreiraBuild prompts and implementation plans for cinematic websites that use GSAP ScrollTrigger, scroll storytelling, pinned heroes, parallax, Three.js scenes, GLB assets, custom shaders, and premium loading screens. Use when Codex needs to design or prompt a landing page, multi-section microsite, or interactive brand experience with exact motion specs, responsive behavior, fixed-canvas 3D effects, or a repeatable framework for prompt-based site builders.
- ▌ Digital Avatar Shopping Video · gabrielmoreira bundle电商运营与自媒体创作者在需要制作商品推荐或促销带货短视频时,使用此技能可一键生成“小省导购员”数字人口播视频。系统自动协同五大智能体完成脚本、画面与音画合成,产出适配抖音、快手等平台的成品,高效搞定带货视频制作。
- ▌ Historical Science Video Prod · gabrielmoreira bundle自媒体创作者与内容创作者在制作历史科学类3分钟科普短视频时,使用此技能可一键生成包含口播文案、分镜脚本、Veo2提示词及人物规范的全流程素材包,完美适配即梦平台,让视频创作更高效。
- ▌ AWS Lambda Durable Functions · gabrielmoreiraBuild resilient, long-running, multi-step applications with AWS Lambda durable functions with automatic state persistence, retry logic, and orchestration for long-running executions. Covers the critical replay model, step operations, wait/callback patterns, error handling with saga pattern, testing with LocalDurableTestRunner. Triggers on phrases like: lambda durable functions, workflow orchestration, state machines, retry/checkpoint patterns, long-running stateful Lambda functions, saga pattern, human-in-the-loop callbacks, and reliable serverless applications.
- ▌ AWS Lambda Managed Instances · gabrielmoreiraEvaluate, configure, and migrate workloads to AWS Lambda Managed Instances (LMI). Triggers on: Lambda Managed Instances, LMI, capacity provider, multi-concurrency Lambda, dedicated instance Lambda, EC2-backed Lambda, cold start elimination, Graviton Lambda, instance type for Lambda, scheduled scaling for LMI, Lambda cost optimization with Reserved Instances or Savings Plans. Also trigger when users describe high-volume predictable workloads seeking cost savings, want to scale LMI capacity on a schedule, or compare Lambda vs EC2 for steady-state traffic. For standard Lambda without LMI, use the aws-lambda skill instead.
- ▌ Business Continuity · gabrielmoreiraDevelop business continuity plans and impact analysis. Implement BCP testing and communication procedures. Use when building organizational resilience.
- ▌ Incident Management · gabrielmoreiraImplement incident management processes and escalation procedures. Configure on-call schedules and post-incident reviews. Use when managing production incidents.
- ▌ Iso27001 Compliance · gabrielmoreiraImplement ISO 27001 Information Security Management System. Configure ISMS controls and risk management. Use when implementing enterprise security frameworks.
- ▌ Database Backups · gabrielmoreiraImplement database backup strategies. Configure automated backups, retention, and recovery testing. Use when designing backup and recovery procedures.
- ▌ Performance Tuning · gabrielmoreiraOptimize Linux system performance. Configure kernel parameters, analyze bottlenecks, and tune resources. Use when improving system performance.
- ▌ Imaging Mass Cytometry · gabrielmoreira bundleWorkflow for multiplexed imaging or IMC segmentation, phenotyping, and spatial summarization.
- ▌ Sequence And Format Io · gabrielmoreira bundleWorkflow for foundational sequence parsing, conversion, compression handling, and interval-aware file validation.
- ▌ Bio Comparative Genomics Ancestral Reconstruction · gabrielmoreira bundleReconstruct ancestral states at internal phylogenetic nodes for sequences (PAML codeml, IQ-TREE --ancestral, GRASP, FastML), discrete traits (corHMM hidden-rate Markov, ape::ace, phytools::make.simmap stochastic mapping, BayesTraits), and continuous traits (phytools::fastAnc, geiger Brownian/OU, RPANDA). Use when designing constructs for ancestral protein resurrection, tracing trait evolution along a tree, performing stochastic character mapping, testing models of trait evolution (BM vs OU vs EB), inferring ancestral genome content via Dollo or DTL reconciliation, or quantifying ancestral-state uncertainty for downstream comparative analyses.
- ▌ Bio Workflows Causal Genomics Pipeline · gabrielmoreira bundleEnd-to-end post-GWAS causal inference pipeline orchestrating heritability partitioning, genetic correlation, Mendelian randomization with CHP-aware sensitivity (CAUSE / LHC-MR), colocalization, fine-mapping with SuSiE / FOCUS, mediation, TWAS triangulation, cis-pQTL drug-target MR, effector-gene prioritization (L2G / PoPS / cS2G), and GenomicSEM common-factor GWAS. Use when triangulating causal inference across multiple complementary methods, prioritizing tissues via stratified LDSC, nominating or de-risking drug targets, mapping a lead SNP to a candidate effector gene, modeling shared genetic architecture across correlated traits, or producing a STROBE-MR-compliant publication-grade evidence battery from GWAS summary statistics.
- ▌ Bio Clip Seq Crosslink Site Detection · gabrielmoreira bundleDetect single-nucleotide crosslink (CL) sites in CLIP-seq data using truncation patterns (iCLIP/eCLIP CITS), crosslink-induced mutations (HITS-CLIP CIMS deletions, PAR-CLIP T-to-C), or HMM/kernel-density methods (PureCLIP, PARalyzer, CTK). Use when single-nucleotide resolution is required for motif registration (mCross), allele-specific binding (BEAPR), variant-effect prediction, or comparing crosslink chemistry across CLIP variants.
- ▌ Bio Methylation Differential Cpg · gabrielmoreira bundlePer-CpG differential methylation testing from bisulfite sequencing count data or beta-value matrices. Covers beta and M-value computation, coverage filtering, statistical tests (Welch t-test, Mann-Whitney, limma, DSS beta-binomial), multiple testing correction, and effect size calculation. Use when comparing methylation at individual CpG sites between experimental groups from WGBS, RRBS, or targeted bisulfite sequencing.
- ▌ Bio Pathway Enrichment Visualization · gabrielmoreira bundleVisualize enrichment results using enrichplot package functions. Use when creating publication-quality figures from clusterProfiler results. Covers dotplot, barplot, cnetplot, emapplot, gseaplot2, ridgeplot, and treeplot.
- ▌ Bio Variant Calling Filtering Best Practices · gabrielmoreira bundleComprehensive variant filtering including GATK VQSR, hard filters, bcftools expressions, and quality metric interpretation for SNPs and indels. Use when filtering variants using GATK best practices.
- ▌ Bio Free Energy Calculations · gabrielmoreira bundlePerforms alchemical free-energy calculations including relative binding free energy (RBFE / FEP+) and absolute binding free energy (ABFE) via OpenFE, FEP+, GROMACS, AMBER pmemd, and OpenMM with explicit lambda window scheduling, soft-core potentials, REST2 enhanced sampling, MBAR/BAR analysis, and cycle closure validation. Compares ML alternatives (Boltz-2 affinity, DeepDock). Use when ranking analogs by binding affinity beyond docking accuracy, performing prospective lead optimization, or validating SAR predictions.
- ▌ Bio Workflows Genome Assembly Pipeline · gabrielmoreira bundleEnd-to-end genome assembly workflow from reads to polished assembly with QC. Supports short reads (SPAdes), long reads (Flye), and hybrid approaches. Use when assembling genomes from raw reads.
- ▌ Bio Systems Biology Metabolic Reconstruction · gabrielmoreira bundleBuild genome-scale metabolic models from genome sequences using CarveMe and gapseq for automated reconstruction. Generate draft models ready for curation and analysis. Use when creating metabolic models for organisms without existing models.
- ▌ Bio Single Cell Metabolite Communication · gabrielmoreira bundleAnalyze metabolite-mediated cell-cell communication using MeboCost for metabolic signaling inference between cell types. Predict metabolite secretion and sensing patterns from scRNA-seq data. Use when studying metabolic crosstalk between cell populations or metabolite-receptor interactions.
- ▌ Bio Clinical Biostatistics Missing Data · gabrielmoreira bundleImplements missing-data sensitivity analyses for confirmatory clinical trials including MMRM under MAR (with Kenward-Roger correction), reference-based multiple imputation (J2R, CR, CIR, LMCF per Carpenter-Roger 2013), Permutt delta-adjustment / tipping-point analysis, pattern-mixture identifying restrictions (CCMV, NCMV, ACMV), and the Cro vs Bartlett variance debate. Use when handling missing primary or secondary endpoint data in regulatory submissions following NRC 2010 and ICH E9(R1).
- ▌ Bio Workflows Somatic Variant Pipeline · gabrielmoreira bundleEnd-to-end somatic variant calling from tumor-normal paired samples using Mutect2 or Strelka2. Covers preprocessing, variant calling, filtering, and annotation for cancer genomics. Use when calling somatic mutations from tumor-normal pairs.
- ▌ Bio Comparative Genomics Whole Genome Duplication · gabrielmoreira bundleDetect, date, and contextualize whole-genome duplication (WGD / paleopolyploidy) events using wgd v2 (Chen & Zwaenepoel 2024), KsRates (Sensalari 2022 substitution-rate-corrected Ks dating), DupGen_finder (Qiao 2019), MAPS (Li 2018 phylogenomic), POInT (Conant 2008 ordered-block), SLEDGe (2024 ML-based), Whale.jl (Bayesian DTL+WGD), and synteny-anchored paranome construction. Use when identifying ancient polyploidy from Ks distributions and synteny block analysis, positioning WGD events relative to speciation, distinguishing tandem from segmental from WGD duplications, dating the 2R/3R vertebrate / fish / salmonid WGDs, building paranome and Ks-age mixture models, applying KsRates substitution-rate correction across lineages, or testing alternative biased-fractionation / dosage-balance models post-WGD.
- ▌ Scientific Impact Assessment · gabrielmoreira bundleAssess paper and journal impact using OpenAlex citation counts, optional Altmetric data, and curated journal impact-factor references. Use when comparing papers, journals, or literature shortlists by reach and influence.
- ▌ Alphafold Database Access · gabrielmoreira bundleAccess AlphaFold DB's 200M+ predicted structures by UniProt ID. Download PDB/mmCIF, analyze pLDDT/PAE, bulk-fetch proteomes via Google Cloud. For experimental structures use PDB; for prediction use ColabFold or ESMFold.
- ▌ Fastp Fastq Preprocessing · gabrielmoreiraAll-in-one FASTQ QC and adapter trimming. Auto-detects Illumina adapters, filters low-quality reads, corrects paired-end overlaps, emits HTML+JSON QC in one pass. 3-10x faster than Trim Galore/Trimmomatic. First step before STAR, BWA-MEM2, or Salmon.
- ▌ Matchms Spectral Matching · gabrielmoreira bundleMS spectral matching and metabolite ID with matchms. Import spectra (mzML, MGF, MSP, JSON), filter/normalize peaks, score similarity (cosine, modified cosine, fingerprint), build reproducible pipelines, identify unknowns vs spectral libraries. Use pyopenms for full LC-MS/MS proteomics.
- ▌ Salmon Rna Quantification · gabrielmoreiraUltra-fast RNA-seq transcript/gene quantification via quasi-mapping (no BAM). Builds a k-mer index from transcriptome FASTA, quantifies in minutes. Outputs TPM/count tables (quant.sf) with optional GC- and sequence-bias correction. Integrates with tximeta/tximport for DESeq2/edgeR. Use STAR when a genome-aligned BAM is needed.
- ▌ Shap Model Explainability · gabrielmoreira bundleModel interpretability via SHAP (Shapley values from game theory). Covers explainer choice (Tree, Deep, Linear, Kernel, Gradient, Permutation), feature attribution, and plots (waterfall, beeswarm, bar, scatter, force, heatmap). Use to explain ML predictions, rank features, debug models, audit fairness, or compare models. Works with tree, deep, linear, and black-box models.
- ▌ Snpeff Variant Annotation · gabrielmoreiraAnnotate and filter VCF variants with SnpEff and SnpSift. SnpEff predicts functional effects (HIGH/MODERATE/LOW/MODIFIER), genes, transcripts, AA changes, HGVS; SnpSift filters and adds ClinVar/dbSNP. Java CLI with Python subprocess integration. Use ANNOVAR for multi-database annotation; Ensembl VEP for REST API; SnpEff for fast CLI with pre-built genomes.
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- ▌ Component Flattening Analysis · gabrielmoreira bundleDetects misplaced classes and fixes component hierarchy problems — finds code that should belong inside a component but sits at the root level. Use when asking "clean up component structure", "find orphaned classes", "fix module hierarchy", "flatten nested components", or analyzing why namespaces have misplaced code. Do NOT use for dependency analysis (use coupling-analysis) or domain grouping (use domain-identification-grouping).
- ▌ Coding Agent Sessions · gabrielmoreira bundleFinds, reads, and reconstructs coding-agent sessions across Codex, Claude, OpenCode, OMO/Senpi, and other local agent logs. Use when asked to find or search past sessions, transcripts, or subagent runs, or to recover what an earlier session did.
- ▌ Prompt Version Management · gabrielmoreiraManage prompt versions, run A/B tests across agent prompts, track performance regressions, and safely roll out prompt changes in production. Covers prompt diffing, semantic versioning, canary releases, and automated evaluation.
- ▌ X Twitter Automation · gabrielmoreiraDesign safe X/Twitter automation workflows for tweet search, reply reads, monitoring, posting, and agent-operated social media actions
- ▌ Habit Formation · gabrielmoreiraAtomic habits, cue-routine-reward loops, habit stacking, accountability systems, and behavior change