Coding & Dev Tools

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Calculate sequence statistics (N50, length distribution, GC content, summary reports) using Biopython. Use when analyzing sequence datasets, generating QC reports, or comparing assemblies.

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Quality control and normalization for metabolomics data. Covers QC-based correction, batch effect removal, and data transformation methods. Use when correcting technical variation in metabolomics data before statistical analysis.

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轻量级全平台 RSS 订阅管理器。一键聚合 YouTube、Vimeo、Behance、B站、微博、抖音、小红书的内容更新，支持增量去重和 AI 智能分类。

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Calculate translation efficiency (TE) as the ratio of ribosome occupancy to mRNA abundance. Use when comparing translational regulation between conditions or identifying genes with altered translation independent of transcription.

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copier-astral is an opinionated Copier template for bootstrapping Python projects using Astral's modern toolchain. It provides batteries-included project scaffolding with linting (ruff), type...

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A/B test video titles for maximum CTR using proven formulas and psychological triggers

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Automatically publish and distribute content across YouTube, TikTok,...

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Convert video scripts to teleprompter format with auto-scroll, timing marks, and delivery coaching

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Bead-based normalization for CyTOF and high-parameter flow cytometry. Covers EQ bead normalization, signal drift correction, and batch normalization. Use when correcting instrument drift in CyTOF or harmonizing data across batches.

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Query NCBI Gene Expression Omnibus (GEO) for expression datasets using Biopython Bio.Entrez. Use when finding microarray/RNA-seq datasets, downloading expression data, or linking GEO series to SRA runs.

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SDLC Layer Separation Architecture — Skill Candidates

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BioServices: Complete Services Reference

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Goal Setting Frameworks for Treatment Plans

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Implicit Constraints

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Approve Implementation

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Troubleshooting Guide

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Guide for choosing Protocol, TypeVar, ParamSpec, or concrete types in stdlib-only scripts

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Predict B-cell and T-cell epitopes using BepiPred, IEDB tools, and structure-based methods for vaccine and antibody design. Identify immunogenic regions in antigens. Use when designing vaccines, mapping antibody binding sites, or predicting immunogenic peptides.

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Systematic problem-solving with Good Teacher mode (default). AI guides through questions, not answers - inspired by Polya's "How to Solve It". Use when user needs to: (1) Solve problems methodically, (2) Learn to think through challenges, (3) Develop problem-solving skills, (4) Find root cause of issues. Triggers: "problem", "solve", "stuck", "how do I", "figure out", "analyze", "debug", "decide", "แก้ปัญหา", "ช่วยคิด", "ติดปัญหา"

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Gather modern best practices and pitfalls for the requested change.

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Portfolio analysis and optimization using OpenBB - performance tracking, risk metrics, asset allocation, rebalancing

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Assess code quality and implement refactorings for TDD team

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Expert PHP code refactoring specialist. Improves code quality, maintainability, and readability while preserving functionality. Applies clean code principles, SOLID patterns, and modern PHP 8.3+ best practices for Laravel and Symfony. Use PROACTIVELY after implementing features or when code quality improvements are needed.

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Guided setup wizard for complete FairDB VPS configuration from scratch

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Multi-Hop Reasoning

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This skill generates a structured chapter outline for intelligent textbooks by analyzing course descriptions, learning graphs, and concept dependencies. Use this skill after the learning graph has been created and before generating chapter content, to design an optimal chapter structure that respects concept dependencies and distributes content evenly across 6-20 chapters.

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Outcome Analysis and Statistical Methods Guide

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Diagnoses and fixes conflicts between Tailscale and proxy/VPN tools (Shadowrocket, Clash, Surge) on macOS. Covers three conflict layers - (1) route hijacking (proxy TUN overrides Tailscale routes), (2) HTTP proxy env var interception (http_proxy/NO_PROXY misconfiguration), and (3) system proxy bypass (browser goes through VPN proxy, DIRECT rule can't reach Tailscale utun). Includes SOP for remote development via SSH tunnels with proxy-safe Makefile patterns. Use when Tailscale ping works but SSH/HTTP times out, when browser returns 503 but curl works, when setting up Tailscale SSH to WSL instances, or when bootstrapping remote dev environments over Tailscale.

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专为技术实战教程设计的公众号文章生成器,遵循小灰灰公众号写作规范,自动生成包含前言、项目介绍、部署实战、总结的完整技术文章,配有详细操作步骤、代码示例,并通过 Gemini-3-Pro-Image-Preview 模型生成配图上传至腾讯云COS图床

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Best Practices for Scientific Diagrams

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BigQuery Guide for IDC

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Bubble Detection Quick Reference (English)

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Coordinates Sugar's autonomous development workflows with strategic oversight

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Guide for how to develop Go apps and modules/libraries. Always use this skill when reading or writing Go code.

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Reviews ExUnit test code for proper patterns, boundary mocking with Mox, and test adapter usage. Use when reviewing _test.exs files or test helper configurations.

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Provides patterns for enabling and operating the Spring Cache abstraction in Spring Boot. Use when implementing application-level caching for performance-sensitive workloads.