Opentrons Workflow

Opentrons Workflow

Use this skill for Opentrons OT-2 workflow guidance and protocol generation.

Workflows

Example P Shape (example)

Use for creating a P-shaped liquid pattern on an Opentrons OT-2 destination plate.

Capabilities:

• Generates an OT-2 Python protocol that dispenses into a fixed set of wells shaped like the letter P
• Uses explicit pipette.aspirate(...) and pipette.dispense(...) calls for each destination well
• Supports configurable source labware, destination labware, tip rack, pipette, deck slots, source well, and dispense volume
• Ends with pipette.drop_tip() so no tip remains attached

Use this workflow when:

• The user wants an example Opentrons workflow
• The task mentions making a P shape, letter pattern, or well-plate pattern using aspirate and dispense
• The workflow should demonstrate direct Opentrons liquid handling rather than optimization

Before running:

• Refer to: example P shape
• Protocol generator: scripts/p_shape.py
• Machine reference: opentrons machine
• Ask the user to confirm all deck slots before generating or running the protocol

Critical Rules

1. Ask the user for source labware, destination labware, tip rack, pipette, mount, deck slots, source well, and dispense volume before generating a runnable protocol.
2. Do not assume deck slots.
3. Use explicit pipette.aspirate(...) and pipette.dispense(...) calls for the P-shape workflow; do not replace them with transfer() or distribute().
4. Opentrons protocols must always end with no tip attached.
5. Do not run robot movement unless the user explicitly approves execution.